5ZTB
Structure of Sulfurtransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.622, 82.770, 138.495 |
| Unit cell angles | 90.00, 115.86, 90.00 |
Refinement procedure
| Resolution | 45.901 - 2.200 |
| R-factor | 0.2168 |
| Rwork | 0.215 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.901 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 84554 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.15 M ammonium sulfate, 0.1 M Tris pH 8.0, 15%(w/v) PEG 4000 |
