5ZT8
SirB from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-06 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.690, 53.370, 80.430 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 38.766 - 2.000 |
| R-factor | 0.1868 |
| Rwork | 0.185 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zt7 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.766 | 38.766 | 2.100 |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.067 | 0.014 | 0.669 |
| Rmeas | 0.080 | 0.017 | 0.799 |
| Number of reflections | 38718 | 579 | 10174 |
| <I/σ(I)> | 14.48 | 61.23 | 1.93 |
| Completeness [%] | 99.3 | 96.8 | 98.9 |
| Redundancy | 3.448 | 3.634 | 3.307 |
| CC(1/2) | 0.998 | 1.000 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1 M CHES, 20% (w/v) PEG 8000 |
