5ZT3

Crystal structure of WA352 from Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	33.849, 35.435, 95.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.458 - 1.304
R-factor	0.1881
Rwork	0.188
R-free	0.19320
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.735
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.330
High resolution limit [Å]	1.300	1.300
Number of reflections	192774
<I/σ(I)>	12.1
Completeness [%]	98.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3000, Calcium acetate, Tris base/ Hydrochloric acid