5ZS6
Dodecameric structure of a small Heat Shock Protein from Mycobacterium marinum M: Form-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 91.290, 91.290, 91.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.550 - 2.812 |
| R-factor | 0.202519140461 |
| Rwork | 0.199 |
| R-free | 0.25024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gme |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.552 | 2.960 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.206 | 0.627 |
| Rpim | 0.045 | |
| Number of reflections | 3204 | 466 |
| <I/σ(I)> | 12.7 | 5.1 |
| Completeness [%] | 100.0 | 96.2 |
| Redundancy | 21.9 | 22.3 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 0.2 M L-Proline, 0.1 M HEPES pH 7.5 and 24 % PEG 1500 |
