5ZRX

Crystal Structure of EphA2/SHIP2 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	138.283, 43.344, 46.377
Unit cell angles	90.00, 95.35, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.1458
Rwork	0.143
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3KKA and 2K4P
RMSD bond length	0.009
RMSD bond angle	1.224
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.047	0.031	0.624
Rmeas	0.056	0.037	0.736
Rpim	0.029	0.020	0.386
Number of reflections	43485	2009	2131
<I/σ(I)>	14.9
Completeness [%]	99.0	87.5	98.1
Redundancy	3.6	3.4	3.5
CC(1/2)		0.998	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	289	0.2M Succinic acid pH 7.0, 20% w/v Polyethylene glycol 3350