5ZRU
Crystal structure of Agl-KA catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.247, 126.423, 80.540 |
| Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
| Resolution | 19.947 - 1.833 |
| R-factor | 0.1426 |
| Rwork | 0.141 |
| R-free | 0.17120 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 98671 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10% PEG 6000, 0.1M HEPES(pH8.5), 10mM ZnSO4 |
