5ZRI
M. smegmatis antimutator protein MutT2 in complex with 5m-dCMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.953720 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.520, 58.840, 31.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.420 - 1.580 |
| R-factor | 0.17834 |
| Rwork | 0.177 |
| R-free | 0.21116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zrc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.836 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.420 | 1.660 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.090 | 0.512 |
| Number of reflections | 17324 | 2481 |
| <I/σ(I)> | 9.1 | 2.3 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 292 | 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate |
