5ZRD

Tyrosinase from Burkholderia thailandensis (BtTYR) at low pH condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 31 2 1
Unit cell lengths	142.914, 142.914, 256.756
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.880 - 2.300
R-factor	0.1819
Rwork	0.180
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zre
RMSD bond length	0.011
RMSD bond angle	1.596
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.800	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	131667
<I/σ(I)>	39.95
Completeness [%]	97.8
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	22% (v/v) reagent alcohol, 100 mM sodium citrate dibasic, pH 4.2