5ZQM

Crystal structure of human katanin AAA ATPase domain complex with ATPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 65
Unit cell lengths	68.907, 68.907, 138.698
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.234 - 2.900
R-factor	0.24927
Rwork	0.247
R-free	0.28514
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zql
RMSD bond length	0.011
RMSD bond angle	1.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.109	0.069	0.386
Rmeas	0.115	0.072	0.454
Rpim	0.036	0.019	0.231
Total number of observations	64181
Number of reflections	8212	855	768
<I/σ(I)>	11
Completeness [%]	98.5	99.4	91.5
Redundancy	7.8	12.2	3.1
CC(1/2)		0.998	0.083

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.4 M Calcium acetate 20% (v/v) PEG 3,350