5ZPQ
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 9 at 288 K (1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2015-11-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.605, 64.262, 158.870 |
| Unit cell angles | 90.00, 117.03, 90.00 |
Refinement procedure
| Resolution | 39.673 - 1.849 |
| R-factor | 0.1467 |
| Rwork | 0.145 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.096 | 0.033 | 0.639 |
| Rmeas | 0.113 | 0.040 | 0.766 |
| Rpim | 0.060 | 0.021 | 0.418 |
| Total number of observations | 521039 | ||
| Number of reflections | 148084 | 7638 | 7367 |
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.9 | 99.5 | 99.7 |
| Redundancy | 3.5 | 3.5 | 3.2 |
| CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
