5ZPM

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 7 at 288 K (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	288
Detector technology	CCD
Collection date	2015-11-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	192.866, 64.320, 158.903
Unit cell angles	90.00, 116.92, 90.00

Refinement procedure

Resolution	36.675 - 1.650
R-factor	0.1497
Rwork	0.148
R-free	0.17120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.086	0.035	0.680
Rmeas	0.101	0.042	0.800
Rpim	0.053	0.022	0.418
Number of reflections	208626	10744	10332
<I/σ(I)>	8.6
Completeness [%]	99.9	99.6	99.9
Redundancy	3.7	3.5	3.6
CC(1/2)		0.998	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES