5ZPI
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 293 K (3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.133, 64.215, 158.779 |
| Unit cell angles | 90.00, 117.06, 90.00 |
Refinement procedure
| Resolution | 40.349 - 1.747 |
| R-factor | 0.1541 |
| Rwork | 0.153 |
| R-free | 0.17480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.863 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.099 | 0.044 | 0.679 |
| Rmeas | 0.113 | 0.052 | 0.776 |
| Rpim | 0.056 | 0.026 | 0.374 |
| Total number of observations | 724823 | ||
| Number of reflections | 174801 | 9048 | 8646 |
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 99.6 | 99.6 | 99.2 |
| Redundancy | 4.1 | 3.8 | 4.2 |
| CC(1/2) | 0.997 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
