5ZPI

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 293 K (3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	293
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.133, 64.215, 158.779
Unit cell angles	90.00, 117.06, 90.00

Refinement procedure

Resolution	40.349 - 1.747
R-factor	0.1541
Rwork	0.153
R-free	0.17480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.863
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.780
High resolution limit [Å]	1.750	4.750	1.750
Rmerge	0.099	0.044	0.679
Rmeas	0.113	0.052	0.776
Rpim	0.056	0.026	0.374
Total number of observations	724823
Number of reflections	174801	9048	8646
<I/σ(I)>	7.5
Completeness [%]	99.6	99.6	99.2
Redundancy	4.1	3.8	4.2
CC(1/2)		0.997	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES