5ZPA

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 283 K (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	283
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.664, 64.991, 158.851
Unit cell angles	90.00, 117.09, 90.00

Refinement procedure

Resolution	35.355 - 1.689
R-factor	0.1606
Rwork	0.159
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1iu7
RMSD bond length	0.006
RMSD bond angle	0.857
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.720
High resolution limit [Å]	1.690	4.590	1.690
Rmerge	0.158	0.094	0.732
Rmeas	0.182	0.108	0.846
Rpim	0.089	0.054	0.419
Total number of observations	796272
Number of reflections	196807	10144	9772
<I/σ(I)>	9.8
Completeness [%]	100.0	99.8	100
Redundancy	4	3.9	4
CC(1/2)		0.985	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES