5ZP6

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 277 K (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	277
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.849, 64.794, 158.843
Unit cell angles	90.00, 117.18, 90.00

Refinement procedure

Resolution	35.324 - 1.688
R-factor	0.1579
Rwork	0.157
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.844
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.720
High resolution limit [Å]	1.690	4.590	1.690
Rmerge	0.184	0.119	0.823
Rmeas	0.208	0.135	0.940
Rpim	0.096	0.062	0.449
Total number of observations	827729
Number of reflections	195405	10221	9604
<I/σ(I)>	9.4
Completeness [%]	97.8	99.2	96.3
Redundancy	4.2	4	4.2
CC(1/2)		0.980	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES