5ZOP

Crystal structure of histone deacetylase 4 (HDAC4) in complex with a SMRT corepressor SP2 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	131.998, 131.998, 67.786
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.435 - 2.698
R-factor	0.2171
Rwork	0.216
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vqw
RMSD bond length	0.002
RMSD bond angle	0.571
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.698	2.700
Number of reflections	18178	1745
<I/σ(I)>	7
Completeness [%]	95.6
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	295	PEG 3350, iso-propanol