5ZON
Histidinol phosphate phosphatase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.030, 71.062, 91.586 |
| Unit cell angles | 92.16, 96.16, 101.94 |
Refinement procedure
| Resolution | 38.500 - 1.940 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.25376 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eq7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.102 | 0.500 |
| Number of reflections | 85945 | 8478 |
| <I/σ(I)> | 10.21 | 2.51 |
| Completeness [%] | 97.6 | 96.7 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.941 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1M ammonium sulfate |
