5ZO9
Eg5 motor domain in complex with STLC-type inhibitor PVEI0021 (C2 type)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.158, 50.729, 94.309 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 19.740 - 2.700 |
| R-factor | 0.23266 |
| Rwork | 0.227 |
| R-free | 0.28318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.578 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.104 | 0.565 |
| Number of reflections | 20540 | 953 |
| <I/σ(I)> | 18.6 | 2.2 |
| Completeness [%] | 98.8 | 94.5 |
| Redundancy | 3.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 24-34% PEG 3350, 0.1M MES, 0.2M Ammonium Sulfate |
