5ZNG
The crystal complex of immune receptor RGA5A_S of Pia from rice (Oryzae sativa) with rice blast (Magnaporthe oryzae) effector protein AVR1-CO39
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-17 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.721, 66.721, 108.328 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.405 - 2.189 |
| R-factor | 0.1635 |
| Rwork | 0.162 |
| R-free | 0.19660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2myv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.886 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 2.268 |
| High resolution limit [Å] | 2.108 | 2.189 |
| Number of reflections | 215689 | |
| <I/σ(I)> | 10.11 | |
| Completeness [%] | 1.0 | |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.1 M ammonium tartrate dibasic 0.1 M sodium acetate-HCl, pH 4.6 |
