5ZN7
Crystal structure of GH31 alpha-xylosidase from a soil metagenome complexed with xylose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-22 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 173.915, 124.865, 177.716 |
Unit cell angles | 90.00, 116.98, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.16672 |
Rwork | 0.165 |
R-free | 0.19065 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.207 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.244 |
Number of reflections | 371318 | 15857 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 93.8 | 80.5 |
Redundancy | 3.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1.6 M lithium sulfate, 100 mM Tris-HCl |