5ZN6
Crystal structure of GH31 alpha-xylosidase from a soil metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 173.146, 124.174, 177.453 |
| Unit cell angles | 90.00, 116.81, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.17525 |
| Rwork | 0.174 |
| R-free | 0.19497 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.252 |
| Number of reflections | 561527 | 23616 |
| <I/σ(I)> | 15.9 | 2.8 |
| Completeness [%] | 91.2 | 77.2 |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1.6 M lithium sulfate, 100 mM Tris-HCl |
