5ZN4

X-ray structure of protein kinase ck2 alpha subunit H148N mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	58.590, 45.531, 63.276
Unit cell angles	90.00, 111.99, 90.00

Refinement procedure

Resolution	33.772 - 1.651
R-factor	0.1991
Rwork	0.197
R-free	0.23860
RMSD bond length	0.006
RMSD bond angle	0.993
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.772	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.039	0.336
Number of reflections	37338	3711
<I/σ(I)>	21.5	2.5
Completeness [%]	99.8	99.7
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M Tris-HCl, 1.2M ammonium sulfate, 5% acetonitrile, 2mM DTT