5ZMW
Crystal structure of the E309Q mutant of SR Ca2+-ATPase in E2(TG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 260.977, 86.895, 60.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.928 - 2.500 |
| R-factor | 0.2234 |
| Rwork | 0.222 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.532 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((dev_3311: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 47627 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 98.8 | |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 16% glycerol, 16-18% PEG2000, 2.5 mM NaN3, 1 mM MgSO4, 1 mM EGTA, 0.15% decylmaltoside and 40 mM MES, pH 6.1 |
