5ZMV

Crystal structure of the E309A mutant of SR Ca2+-ATPase in E2(TG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9
Spacegroup name	P 41 21 2
Unit cell lengths	71.200, 71.200, 589.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.976 - 3.300
R-factor	0.1963
Rwork	0.195
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((dev_2341))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.410
High resolution limit [Å]	3.300	3.300
Number of reflections	23965
<I/σ(I)>	15
Completeness [%]	99.5
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298