5ZM9

Crystal structure of hexacoordinated heme protein from anhydrobiotic tardigrade at pH 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-02-21
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	36.312, 56.627, 66.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.890 - 2.700
R-factor	0.21969
Rwork	0.217
R-free	0.26679
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.175
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.163	0.619
Number of reflections	4057
<I/σ(I)>	13
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)		0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG 400, sodium chloride, HEPES