5ZM7

Crystal structure of ORP1-ORD in complex with cholesterol at 3.4 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 41 3 2
Unit cell lengths	149.253, 149.253, 149.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.199 - 3.401
R-factor	0.2782
Rwork	0.277
R-free	0.31230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ic4
RMSD bond length	0.009
RMSD bond angle	0.581
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.400	3.401
Rmerge	0.074	0.549
Rmeas	0.076	0.565
Rpim	0.018	0.133
Number of reflections	8299	793
<I/σ(I)>	41.67	8.11
Completeness [%]	99.8	100
Redundancy	17.9	18.1
CC(1/2)		0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100mM Sodium acetate, pH 4.6, 2.0M Sodium formate