5ZM2

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AndA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-04-29
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 1
Unit cell lengths	61.954, 74.887, 87.450
Unit cell angles	103.95, 108.22, 90.07

Refinement procedure

Resolution	47.078 - 2.500
R-factor	0.2004
Rwork	0.198
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybm
RMSD bond length	0.008
RMSD bond angle	1.002
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.550	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.149	0.511
Number of reflections	48298	4442
<I/σ(I)>	6.6
Completeness [%]	96.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% (w/v) PEG 3350, 200mM sodium tartrate, 20% DMF