5ZKN

Structure of N-acetylmannosamine-6-phosphate 2-epimerase from Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	I 2 2 2
Unit cell lengths	47.515, 75.347, 135.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.820 - 2.205
R-factor	0.1856
Rwork	0.183
R-free	0.23260
RMSD bond length	0.009
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.850	44.850	2.270
High resolution limit [Å]	2.210	9.090	2.210
Rmerge	0.070	0.044	0.700
Rmeas	0.076	0.048	0.761
Rpim	0.030	0.020	0.297
Total number of observations	80912	1075	6997
Number of reflections	12687	213	1086
<I/σ(I)>	13.5	32.4	2.8
Completeness [%]	99.9	98.4	99
Redundancy	6.4	5	6.4
CC(1/2)	0.998	0.997	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	20% PEG 3350, 0,1M MES pH5.2, 0.4M MgCl2