5ZKK
Crystal structure of Phosphoserine phosphatase from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-08-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 109.172, 109.172, 165.911 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.550 - 2.000 |
| R-factor | 0.16823 |
| Rwork | 0.167 |
| R-free | 0.20193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ij5 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.550 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39785 | 2002 |
| <I/σ(I)> | 27.6 | 3.4 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 14.6 | 13.8 |
| CC(1/2) | 0.996 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 289.15 | 0.1 M phosphate citrate buffer, pH 4.2, 4% PEG 300 |
