5ZKI

Human EXOG-H140A in complex with duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	80
Detector technology	CCD
Collection date	2017-06-01
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	73.497, 91.123, 129.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.619 - 2.321
R-factor	0.1816
Rwork	0.180
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a1n
RMSD bond length	0.003
RMSD bond angle	0.515
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.370
High resolution limit [Å]	2.320	2.320
Rmerge	0.078	0.479
Rmeas	0.084	0.517
Rpim	0.032	0.194
Number of reflections	38137	2468
<I/σ(I)>	19.49	3.794
Completeness [%]	99.8	99.3
Redundancy	7	6.5
CC(1/2)	0.986	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	0.1M BIS-Tris pH 6.5, 20% w/v PEG 1500