5ZJU
Crystal structure of in vitro expressed and assembled PCV2 Virus-like Particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17A |
| Synchrotron site | NSRRC |
| Beamline | BL17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 194.116, 201.877, 231.281 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.1657 |
| Rwork | 0.163 |
| R-free | 0.22408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r0r |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 432268 | |
| <I/σ(I)> | 13.5 | 2.31 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulfate, Citrate |
