5ZJT

Structure of AbdB/Exd complex bound to a 'Black14' DNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	45.440, 45.630, 66.860
Unit cell angles	99.05, 100.37, 114.18

Refinement procedure

Resolution	63.551 - 2.400
R-factor	0.2367
Rwork	0.232
R-free	0.27590
RMSD bond length	0.003
RMSD bond angle	0.468
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.551	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	16923
<I/σ(I)>	6.8
Completeness [%]	93.1
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M Sodium citrate pH 5.3, 25% PEG 3350