5ZJR
Structure of AbdB/Exd complex bound to a 'Magenta14' DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.330, 49.445, 95.092 |
| Unit cell angles | 90.00, 109.18, 90.00 |
Refinement procedure
| Resolution | 44.906 - 3.030 |
| R-factor | 0.2697 |
| Rwork | 0.269 |
| R-free | 0.28800 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.552 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.906 | 3.139 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Number of reflections | 6678 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 90mM MgCl2, 0.1M Tris pH9.0, 22% PEG 3350 |
