5ZJK

Structure of myroilysin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.977
Spacegroup name	P 1 21 1
Unit cell lengths	125.176, 115.902, 163.354
Unit cell angles	90.00, 93.71, 90.00

Refinement procedure

Resolution	19.993 - 2.600
R-factor	0.19
Rwork	0.187
R-free	0.25370
RMSD bond length	0.003
RMSD bond angle	0.693
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.740
High resolution limit [Å]	2.600	2.600
Rpim	0.071	0.317
Number of reflections	142484	20724
<I/σ(I)>	9.4	2.5
Completeness [%]	99.5	99.5
Redundancy	7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	potassium phosphate