5ZJG

Gamma-glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly-Gly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.96401
Spacegroup name	P 1
Unit cell lengths	43.537, 53.942, 107.260
Unit cell angles	91.45, 100.19, 108.08

Refinement procedure

Resolution	20.067 - 1.702
R-factor	0.1555
Rwork	0.155
R-free	0.18920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dg5
RMSD bond length	0.007
RMSD bond angle	0.924
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.052	0.520
Rmeas	0.055	0.567
Rpim	0.020	0.221
Number of reflections	121960	5713
<I/σ(I)>	26.3
Completeness [%]	96.6	90.6
Redundancy	7.5	6
CC(1/2)	0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	16% (w/v) PEG 8000, 15% (w/v) PEG 400, 0.1M HEPES (pH 7.0), 50mM glycylglycine