5ZIN

Crystal structure of bacteriorhodopsin at 1.27 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	15
Detector technology	PIXEL
Collection date	2017-04-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8
Spacegroup name	P 63
Unit cell lengths	60.630, 60.630, 110.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.000 - 1.270
Rwork	0.130
R-free	0.16310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
Data scaling software	XSCALE
Refinement software	SHELXL

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.000	47.000	1.300
High resolution limit [Å]	1.270	5.680	1.270
Rmerge	0.133	0.050	4.331
Rmeas	0.134	0.050	4.359
Number of reflections	60573	702	4489
<I/σ(I)>	22.25	97.35	1.09
Completeness [%]	100.0	99.7	100
Redundancy	81.457	86.396	77.366
CC(1/2)	1.000	1.000	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	MO, 2.0-2.5 M phosphate