5ZIL

Crystal structure of bacteriorhodopsin at 1.29 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	15
Detector technology	PIXEL
Collection date	2017-10-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8
Spacegroup name	P 63
Unit cell lengths	60.590, 60.590, 110.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.421 - 1.290
Rwork	0.152
R-free	0.16400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
Data scaling software	XDS
Refinement software	SHELX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.421	47.421	1.370
High resolution limit [Å]	1.290	3.860	1.290
Rmerge	0.062	0.032	0.944
Rmeas	0.069	0.035	1.044
Total number of observations	320609
Number of reflections	57755	2209	9249
<I/σ(I)>	13.41	43.44	1.76
Completeness [%]	99.9	99.6	99.7
Redundancy	5.551	5.293	5.509
CC(1/2)	0.999	0.999	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	293	MO, 2.0-2.5 M PHOSPHATE