5ZIK
Crystal structure of Ketopantoate reductase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2017-07-19 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97949 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 131.310, 131.310, 156.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.123 - 2.450 |
R-factor | 0.2244 |
Rwork | 0.224 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ks9 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.444 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.010 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.073 | |
Rpim | 0.024 | 0.394 |
Number of reflections | 51232 | 5014 |
<I/σ(I)> | 24.5 | 2.3 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 10 | 10.2 |
CC(1/2) | 0.999 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 295.15 | 1.1M Sodium-Potassium-Tartarate, 50mM BICINE pH 8.0 |