5ZID
Crystal Structure of human DPP-IV in complex with HL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.070, 126.190, 118.620 |
| Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
| Resolution | 85.820 - 3.000 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3j |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.885 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.820 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.149 | 0.329 |
| Number of reflections | 35139 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 91.2 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100mM Tris pH 8, 18% PEG 3350, 200mM NaCl |
