5ZI9

Crystal structure of type-II LOG from Streptomyces coelicolor A3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-27
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	I 2 3
Unit cell lengths	206.768, 206.768, 206.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	146.210 - 2.500
R-factor	0.1872
Rwork	0.185
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wq3
RMSD bond length	0.015
RMSD bond angle	1.762
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	146.210	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.108	0.310
Number of reflections	49440	2396
<I/σ(I)>	17.67	3.8
Completeness [%]	98.0	96.8
Redundancy	8.7
CC(1/2)		0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Polyethylene glycol 3350, citrate