5ZI6

The RING domain structure of MEX-3C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.977
Spacegroup name	P 1
Unit cell lengths	37.135, 44.567, 67.157
Unit cell angles	92.43, 90.50, 91.39

Refinement procedure

Resolution	36.384 - 2.200
R-factor	0.2088
Rwork	0.207
R-free	0.24340
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.281
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.088	0.052	0.898
Rmeas	0.103	0.060	1.062
Rpim	0.053	0.031	0.562
Number of reflections	20938	1025	973
<I/σ(I)>	4.1
Completeness [%]	94.3	91.7	90.1
Redundancy	3.6	3.7	3.1
CC(1/2)		0.998	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M Ammonium Acetate, 0.1M Bis-Tris pH 6.5, 25%(w/v) PEG3350