5ZHR
Crystal structure of OsD14 in complex with covalently bound KK094
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.250, 88.640, 119.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.511 - 1.450 |
| R-factor | 0.1669 |
| Rwork | 0.166 |
| R-free | 0.19930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vxk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.511 | 1.540 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.047 | 0.274 |
| Number of reflections | 173170 | |
| <I/σ(I)> | 26.3 | 5.9 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 9.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 20000, MES |
