5ZF2

Crystal structure of Trxlp from Edwardsiella tarda EIB202

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97852
Spacegroup name	P 61 2 2
Unit cell lengths	34.273, 34.273, 264.702
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.059 - 1.980
R-factor	0.181
Rwork	0.177
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yzu
RMSD bond length	0.009
RMSD bond angle	0.923
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.970	5.350	1.970
Rmerge	0.076	0.048	0.200
Rmeas	0.078	0.049	0.203
Rpim	0.014	0.010	0.037
Number of reflections	7481	499	351
<I/σ(I)>	8.1
Completeness [%]	99.9	99.8	99.7
Redundancy	30.4	25.3	29.3
CC(1/2)		0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	30% PEG 8000, 100mM sodium acetate, pH6.5, 200mM lithium sulfate