5ZES

UDP Glucose alpha tetrahydrobiopterin glycosyltransferase from Synechococcus species PCC 7942 - UDP complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979,0.979,0.987
Spacegroup name	C 1 2 1
Unit cell lengths	171.810, 78.698, 53.605
Unit cell angles	90.00, 90.87, 90.00

Refinement procedure

Resolution	29.010 - 2.030
R-factor	0.1854
Rwork	0.183
R-free	0.23536
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.925
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.030	2.030
Number of reflections	46172
<I/σ(I)>	10
Completeness [%]	99.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Bis-tris, PEG3350, Galactose