5ZEM
Crystal structure of the bacterial A1408me1A-mutant ribosomal decoding site in the presence of gentamicin
Replaces: 4WCSExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 32.584, 91.995, 47.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.300 - 3.103 |
| R-factor | 0.258 |
| Rwork | 0.255 |
| R-free | 0.28350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3td1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.143 |
| Data reduction software | d*TREK |
| Data scaling software | CrystalClear |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.068 | 0.284 |
| Number of reflections | 2851 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, spermine, MPD, Potassium chloride |
