5ZEH
Crystal structure of Entamoeba histolytica Arginase in complex with L- Ornithine at 2.35 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 87.605, 97.494, 133.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.680 - 2.360 |
| R-factor | 0.22082 |
| Rwork | 0.219 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.357 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.680 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 22967 | 2102 |
| <I/σ(I)> | 6.8 | 2.2 |
| Completeness [%] | 96.4 | 90.8 |
| Redundancy | 5.1 | 4.1 |
| CC(1/2) | 0.990 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.3 | 293.15 | 0.1M bistris propane (pH-9.3), 0.2M potassium thiocyanate, 21% Peg6000 |
