Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.700, 46.520, 38.760 |
| Unit cell angles | 90.00, 96.35, 90.00 |
Refinement procedure
| Resolution | 38.500 - 2.400 |
| R-factor | 0.1818 |
| Rwork | 0.178 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j7t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.981 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.042 | 0.330 |
| Number of reflections | 11198 | |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 99.4 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | sodium cacodylate, spermine, MPD, ammonium chloride, |
