5ZDS
Crystal structure of the second PDZ domain of Frmpd2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97858 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 48.176, 56.842, 32.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.2017 |
| Rwork | 0.198 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vj6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.831 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmeas | 0.065 | 0.268 |
| Number of reflections | 12699 | 1248 |
| <I/σ(I)> | 22.52 | 7.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG3350 20%, sodium thiocyanate 0.2M |
