5ZDN
The complex structure of FomD with CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.165, 77.452, 96.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.280 - 2.020 |
| R-factor | 0.19559 |
| Rwork | 0.193 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3exm |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.044 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.068 | 0.525 |
| Number of reflections | 14166 | 1015 |
| <I/σ(I)> | 16.4 | 2.1 |
| Completeness [%] | 99.8 | 97.4 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | Tris-HCl, magnesium chloride, PEG 8000, EtOH, CDP |
