5ZCX

Structure of T20/N39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	39.790, 38.001, 73.080
Unit cell angles	90.00, 101.52, 90.00

Refinement procedure

Resolution	37.535 - 2.299
R-factor	0.2337
Rwork	0.233
R-free	0.24860
RMSD bond length	0.010
RMSD bond angle	0.910
Data scaling software	XDS
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.381
High resolution limit [Å]	2.299	2.299
Number of reflections	9725
<I/σ(I)>	4.51
Completeness [%]	100.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	0.05 M caesium chloride 0.1 M MES pH 6.5 30 % v/v Jeffamine M-600