5ZCG

Crystal structure of OsPP2C50 S265L/I267V:OsPYL/RCAR3 with (+)-ABA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	70.738, 76.351, 77.942
Unit cell angles	62.96, 72.04, 66.93

Refinement procedure

Resolution	32.946 - 2.100
R-factor	0.2028
Rwork	0.202
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gwp
RMSD bond length	0.004
RMSD bond angle	0.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.950	2.041
High resolution limit [Å]	1.970	1.970
Rmerge	0.068	0.332
Rpim	0.046	0.221
Number of reflections	89408	7413
<I/σ(I)>	16.61
Completeness [%]	95.8
Redundancy	3.2
CC(1/2)	0.996	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	7.5% PEG 3350, 80mM ammonium thiocyanate